Composite

Part:BBa_K1175028:Design

Designed by: Nicholas K. Goldner   Group: iGEM13_WLC-Milwaukee   (2013-09-17)


Strong pBAD promoter BBa_K206000 attached to SecI pump BBa_K215104


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1582
    Illegal BsaI.rc site found at 1228
    Illegal BsaI.rc site found at 3832


Design Notes

We had to remove the RBS and in the future will be removing all Bative RBSs from the sequence to be replaced with BBa_B0034


Source

iGEM registry, acquired from University of Washington 2009 iGEM team from the genomic DNA of Erwinia chrysanthemi

References